Brucella Blood Agar (commonly notated as BBA or BRU) is a non-selective agar medium used for isolation, quantitation, and partial identification of fastidious anaerobes. This agar was originally developed for the isolation of Brucella spp. from potentially contaminated materials but has since been found to be useful for use in clinical specimens involving strict anaerobes. This agar is typically supplemented with Hemin and Vitamin K1.
Tryptic Soy Agar or Trypticase Soy Agar, often abbreviated as TSA, is a general purpose, non-selective agar media that supports the growth of a wide-range of microorganisms. TSA can be used for a variety of applications including culture storage, enumeration, and isolation of microorganisms.
Gram staining, also called Gram's Method, is named after Hans Christian Gram who developed the method in 1884, which allows for one to distinguish between gram-postive and gram-negative bacteria using a combination of different chemical stains. In this test, bacteria that retain the crystal violet dye do so because of a thick layer of peptidoglycan. In contrast, negative bacteria do not retain the violet dye and appear as red/pink.