Bile Salt Irgasan Brilliant Green Agar (BSIBG) is a highly selective agar medium that uses bile salts, brilliant green, and Irgasan for the isolation of Aeromonas spp. from a variety of environmental, clinical, and food samples. Aeromonas spp. has been regarded as controversial pathogen and a possible source of food-borne infection with some strains noted as being enteropathogenic. This species of bacteria has also been found to grow and produce virulence factors not only at optimal growth temperature, but at refrigeration temperatures as well.