Standard Time-Kill Testing

A Standard Time-Kill, often referred to as STK, Suspension Test, or Time-Kill Kinetics Assay, is an in vitro test which measures the changes in a population of aerobic microorganisms in a pre-determined sampling time while antimicrobial properties are present.  This test method is often used to measure antimicrobial activity in hand sanitizers, antiseptic wound gels, mouth washes, and other products that are expected to have microbiocidal properties [4]. Standard Time-Kill methods are based on the American Society for Testing and Materials method, Standard Guide for Assessment of Antimicrobial Activity Using a Time-Kill Procedure (E2315-16) [1].