BCA

Bicinchoninic Acid Assay (BCA)

The Bicinchoninic Acid Assay (BCA), also referred to as the Smiths' Assay, is a biochemical assay designed by Paul Smith in 1985. (Smith, et al., [7])  This assay uses colorimetric detection and quantification to determine the total concentration of protein in a solution by exhibiting a measurable color change. Applications of this assay include studying protein-protein interactions, estimating percent recovery of membrane proteins from cell extracts, high-throughput screening of fusion proteins, measuring column fractions after affinity chromatography and measuring protein covalently bound to agarose supports and protein adsorbed to multiwell plates.