Tryptic Soy Agar (TSA)

Introduction

Tryptic Soy Agar or Trypticase Soy Agar, often abbreviated as TSA, is a general purpose, non-selective agar media that supports the growth of a wide-range of microorganisms.  TSA can be used for a variety of applications including culture storage, enumeration, and isolation of microorganisms.

TSA contains digest of soybean meal and casein making it suitable for growth of fastidious (any organism that has a complex nutritional requirement) and non-fastidious microorganisms. The combination of peptones allow for an adequate supply of nitrogen in the form of amino acids and polypeptides while sodium chloride is added to maintain the osmotic equilibrium. 

TSA may be supplemented with additional components to facilitate the growth of more selective microorganisms. 

Reconstitution

The following table shows the weight of each component added to 1 liter of sterile RO/DI water.  Commercialized stocks are sold in which all dry components are pre mixed. The correct weight of commercialized powder to water is 40 g/L.   Regardless of whether components are individually added or mixed prior, the solid components should be allowed to soak for 10 minutes, and then sterilized via an autoclave for atleast 15 minutes at 121°C. 

Tryptic Soy Agar Reconstitution Table

Appearance:  Pale, clear gel. 

pH: 7.3 ± 0.2

Storage

Plates may be stored up to 7 days at 2-8°C away from light

Capped containers may be stored up to 3 months at 15-20°C away from light. 

 Sterilize all biohazard waste before disposal


References

[1] “Microbiology Introduction.” Sigma-Aldrich, The Journal of Pharmacy and Pharmacology, www.sigmaaldrich.com/technical-documents/articles/microbiology/microbiology-introduction.html.

[2] “Microbiology Theory: Media Preparation.” Sigma-Aldrich, The Journal of Pharmacy and Pharmacology, www.sigmaaldrich.com/technical-documents/articles/microbiology/media-preparation.html.

[3] “TRYPTIC SOY AGAR (TSA).” Kligler Iron Agar (KIA) - or the Identificaiton of Enteric Bacteria, catalog.hardydiagnostics.com/cp_prod/Content/hugo/TrypticSoyAgar.htm.

[4] “Tryptic Soy Agar 22091.” Sigma-Aldrich, The Journal of Pharmacy and Pharmacology, www.sigmaaldrich.com/catalog/product/sial/22091?lang=en®ion=US&gclid=Cj0KCQjwx43ZBRCeARIsANzpzb_gFttTWkMEgEc2J0mikvjwFsOBuAcTXC1XibRryMpXu-FWTNyIbQEaAshwEALw_wcB.